Small heat-shock proteins: important players in regulating cellular proteostasis

Small heat-shock proteins (sHsps) are a diverse family of intra-cellular molecular chaperone proteins that play a critical role in mitigating and preventing protein aggregation under stress conditions such as elevated temperature, oxidation and infection. In doing so, they assist in the maintenance of protein homeostasis (proteostasis) thereby avoiding the deleterious effects that result from loss of protein function and/or protein aggregation. The chaperone properties of sHsps are therefore employed extensively in many tissues to prevent the development of diseases associated with protein aggregation. Significant progress has been made of late in understanding the structure and chaperone mechanism of sHsps. In this review, we discuss some of these advances, with a focus on mammalian sHsp hetero-oligomerisation, the mechanism by which sHsps act as molecular chaperones to prevent both amorphous and fibrillar protein aggregation, and the role of post-translational modifications in sHsp chaperone function, particularly in the context of disease.SM was supported by a Royal Society Dorothy Hodgkin Fellowship, HE is supported by an Australian Research Council Future Fellowship (FT110100586) and JC is supported by a National Health and Medical Research Council Project Grant (#1068087)

A case of porphyria cutanea tarda in the setting of hepatitis C infection and tobacco usage

Porphyria cutanea tarda (PCT) is the most common type of porphyria, presenting in middle-aged patients with a photodistributed vesiculobullous eruption, milia, and scars. Porphyria cutanea tarda occurs in relation to inhibition of uroporphyrinogen decarboxylase, a key enzyme in the heme biosynthesis pathway. A number of genetic and acquired factors increase susceptibility to PCT by reducing uroporphyrinogen decarboxylase activity. A handful of other vesiculobullous conditions may mimic PCT both clinically and histologically; therefore, both skin biopsy and laboratory evaluation are helpful in confirming the diagnosis. We report a case of PCT in the setting of cigarette usage and untreated hepatitis C infection

Contrastive grammar : a theory and practice handbook

En consonancia con los lineamientos del programa vigente de Gramática Contrastiva, materia incluida en el programa de estudios del Traductorado de Inglés de la Facultad de Lenguas, Universidad Nacional de Córdoba, el objetivo principal de Contrastive Grammar: A Theory and Practice Handbook es brindar a los estudiantes un manual que combine las gramáticas descriptivas del inglés y del español. No pretende ser una revisión completa de todas las diferencias lingüísticas existentes entre ambas lenguas: por el contrario, el objetivo del presente manual es combinar información teórica clave con prácticas variadas respecto de estructuras dispares que representan la fuente más frecuente de interferencia entre los dos sistemas.Fil: Gómez Calvillo, M. Natalia. Universidad Nacional de Córdoba. Facultad de Lenguas; Argentina.Fil: Meehan, Patricia. Universidad Nacional de Córdoba. Facultad de Lenguas; Argentina.Fil: Díaz, M. Josefina. Universidad Nacional de Córdoba. Facultad de Lenguas; Argentina.Fil: Rolfi, Laura. Universidad Nacional de Córdoba. Facultad de Lenguas; Argentina

A preferred vision for administering elementary schools : a reflective essay

As I reflect upon my vision of effective teaching and leadership within a school, I am energized to implement the many ideas and concepts from this paper into action.· The school administrator plays a pivotal role in establishing the climate and mission for a learning community. My leadership efforts need to be visionary and move the school to its desired state. I must create the atmosphere, with the help of others, that transform the dreams of the learning community into reality. I look forward to stepping into this role someday and impacting the lives and futures of the students, families, and staff that I work with

From Protein Folding to Blood Coagulation: Menaquinone as a Metabolic Link between Bacteria and Mammals

Menaquinones have long played a central role in bacterial metabolism due to their solubility in membranes and their ability to mediate electron transfer reactions between a large variety of enzymes. In addition to acting as important nodes in fermentation and respiration, menaquinones are critical to the formation of disulphide bonds in the periplasm. Their utility as molecular wires has also led to their incorporation into redox reactions in higher‐order organisms, where they participate in numerous physiological processes, including blood coagulation. Through studying the menaquinone‐dependent pathways in organisms across the phylogenetic spectrum, researchers have begun to uncover intriguing metabolic links and have identified novel compounds for modulating these vital pathways

The visual, the auditory and the haptic – A user study on combining modalities in virtual worlds

Fröhlich J, Wachsmuth I. The visual, the auditory and the haptic – A user study on combining modalities in virtual worlds. In: Shumaker R, ed. Virtual Augmented and Mixed Reality. Designing and Developing Augmented and Virtual Environments. Lecture Notes in Computer Science. Vol 8021. Berlin, Heidelberg: Springer Berlin Heidelberg; 2013: 159-168.In order to make a step further towards understanding the impact of multi-modal stimuli in Virtual Reality we conducted a user study with 80 participants performing tasks in a virtual pit environment. Participants were divided into four groups, each presented a different combination of multi-sensory stimuli. Those included real-time 3D graphics, audio stimuli (ambient, static and event sounds), and haptics consisting of wind and tactile feedback when touching objects. A presence questionnaire was used to evaluate subjectively reported presence on the one hand, and on the other physiological sensors were used to measure heart rate and skin conductance as an objective measure. Results strongly indicate that an increase of modalities does not automatically result in an increase of presence

Visual clues act as a substitute for haptic feedback in robotic surgery

Objective: The lack of haptic feedback (HF) in robotic surgery is one of the major concerns of novice surgeons to that field. The superior visual appearances acquired during robotic surgery may give clues that make HF less important. Methods: We surveyed 52 individuals on their perception of HF during robotic surgery. The first group of 34 surgically inexperienced people used the da Vinci robot for their first time (drylab). The second group included 8 laparoscopic surgeons with experience up to a fifth robotic operation. The third group included 10 surgical experts with substantial experience (150-650 robotic cases). Visual analog assessment was made of perception of HF, how much HF was missed, how much the absence of HF impaired the operators' level of comfort. Robotic experts were asked if complications have occurred as a result of a lack of HF. Results: Of the first group, 50% reported the perception of HF, as did 55% of the second group and 100% of the third group (difference between group 1 and group 3: p<0.05). The first group missed HF for 6.5; the second group for 4.3, and the third group for 4 (difference between groups 1 and 3: p<0.05). The surgical experts claimed to have missed HF for 7.2 s when they first started robotic surgery (Difference to now: p<0.05). The lack of HF caused discomfort for the first group of 4; for the second group of 4,4, and for the third group of 2,6. One complication was reported by the robotic experts as resulting from the lack of HF. Conclusions: The data support the conclusion that even beginners quickly experience the perception of HF when performing robotic surgery. With more experience, perception of HF and the level of comfort with robotic surgery increases significantly. This perception of HF makes "real” HF less important and demonstrates that its importance is overestimated by novices in robotic surger